EXPERIMENTAL STUDY OF EFFECTS OF MAGNET-CONTROLLED SORBENT (Fe3O4) PARAMETERS OF ACID-BASE EQUILIBRIUM IN BLOOD AND PROCESSES OF GLYCOLYSIS IN ERYTHROCYTES

A.N. Belousov

Disperse liquid-magnetic media are still wider used in biology and medicine. As a result of solution of various fundamental and applied problems facing bionic principles of application of magnetic fluids, it becomes possible not only to work out but also to receive a new class of magnetic fluids with unique properties enabling their active use in medicine and biology. At present, taking into account advanced scientific nanotechnologies based on synthesis of magnetic fluids, Dr. Belousov has created and patented a new magnet-controlled sorbent (MCS) at the heart which is magnetite - Fe3O4. The technique of extracorporal detoxication with use of a magnetic fluid as a haemosorbent significantly differs from those ones, which exist now. The principle of extracorporal haemocorrection is based on physicochemical properties of this haemosorbent. Colloid particles of the magnetite (Fe3O4), 6-12 nm in size, actively absorb free and protein complex-bound toxic substances, circulating in the blood, on their surfaces. In order to study effects of MCS on parameters of the acid-base equilibrium (ABE) in an experiment, we have investigated indices of pH and alkali reserve (BB) of the human venous blood in vitro as well as revealed the level of end products of glycolysis in erythrocytes (ATP and 2.3 DPG). For an experimental study of effects of MCS on ABE indices in the venous blood, we selected 50 patients with marked signs of intoxication who were in intensive care units with various surgical and therapeutic pathologies. As a result of the investigations, we have reliably revealed that in all the cases, following a single session of haemocorrection with use of Belousov's MCS, in patients with signs of a disturbed ABE there is a process of rapid restoration of indices of pH and BB in the venous blood irrespective of the type of their ABE disturbance. Thus, e.g., in 13 patients with signs of subcompensated respiratory alkalosis and 28 patients with subcompensated metabolic acidosis, indices of pH and BB in vitro after passage of their venous blood through MCS restored to an average statistic range of normal values as early as after the first session of haemocorrection. The obtained data testified to universal abilities of MCS to rapidly correct ABE parameters of the venous blood in various kinds of their disturbances. This fact is a result of interaction of constant magnetic intensity gradient of the MCS colloid particle with the bioelectrical potential of the erythrocyte. In other words, a change of biochemical processes in the erythrocyte should be regarded as a result of action of physical forces, which appear in return to movement of the colloid particle of MCS in the zone of the electrical field of the erythrocyte. It results both in correction of the bioelectrical potential of the value of the charge on the outer and inner walls of the erythrocyte membrane, and a changed activity of intracellular biochemical processes which take place in the erythrocyte itself. The above facts are confirmed by an increased level in the content of end products of glycolysis (ATP and 2,3 DPG) in the erythrocyte by 1.5 - 2 times after correction with MCS in vitro. Thus, MCS in vitro produces a marked stimulating effect on processes of glycolysis taking place in the erythrocyte. In the end, this fact causes an increase in the oxygen capacity of blood and normalization of the membrane potential of the erythrocyte charge. The above circumstance, on the whole, determines the state of activity of the haemoglobin buffer in the venous blood. Finally, this state is the main cause of a rapid and universal correction in ABE parameters of the blood.
 

Keywords: magnet-controlled sorbent MCS-B; extracorporal haemocorrection;  activity of intracellular biochemical processes in the erythrocyte; increase in the oxygen capacity of blood; universal correction in ABE parameters.

 


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